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Objective:To investigate the role of prophylactic cranial irradiation (PCI) in non-small cell lung cancer (NSCLC) by meta-analysis.Methods:Studies published from January 1, 1980 to August 30, 2021 were searched systematically in PubMed, Embase, Cochrane Systematic Review database and China National Knowledge Infrastructure Database. The searching keywords included "non-small cell lung cancer", "randomized controlled trial", "prophylactic cranial irradiation" and "clinical trial". The data extracted from the above studies were analyzed using Review Manager 5.3 and Stata 12.0 software. Outcomes included the development of brain metastases (BM), overall survival (OS), disease-free survival (DFS), toxicity, and quality of life (QoL).Results:Ten trials, including 2005 NSCLC patients, met the inclusion criteria. Patients who underwent PCI had a significantly lower risk of BM than those who did not ( OR=0.29, 95% CI: 0.22-0.40, P<0.001). Compared with non-PCI group, DFS in PCI group was significantly increased ( HR=0.75, 95% CI: 0.63-0.89, P=0.001). However, there was no significant difference in OS ( OR=0.90, 95% CI: 0.69-1.18, P=0.45). In addition, the incidence of fatigue was significantly increased in the PCI group ( OR=2.64, 95% CI: 1.58-4.40, P<0.001). There was no significant difference in cognitive impairment between the PCI and non-PCI groups ( OR=3.60, 95% CI: 0.97-13.32, P=0.06). Conclusions:PCI is the standard treatment for NSCLC. Compared with non-PCI, PCI significantly reduces the incidence of BM and prolongs the DFS of NSCLC patients. The effect of PCI-related toxicity on the QoL and long-term OS needs further study.
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Objective:To analyze the distribution and drug resistance of pathogenic bacteria in skin and soft tissue infections.Methods:The lesion samples were collected from patients with skin and soft tissue infections in the First Affiliated Hospital of Hainan Medical College from January 2012 to September 2021, and the distribution of pathogenic bacteria and their drug resistance were analyzed. The data were analyzed with SPSS 23.0 statistical software.Results:A total of 692 strains of pathogenic bacteria were isolated from 1 121 patients.There were 372 strains of Gram-negative bacteria (53.76%), mainly Pseudomonas aeruginosa ( n=92, 13.29%), Klebsiella pneumoniae ( n=55, 7.95%) and Enterobacter cloacae ( n=38, 5.49%); 276 strains of gram-positive bacteria (39.88%), mainly Staphylococcus aureus ( n=140, 20.23%), Streptococcus pyogenes ( n=38, 5.49%) and Enterococcus faecalis ( n=27, 3.90%); and 44 strains of fungi (6.36%), mainly Candida albicans ( n=19, 2.75%) and Candida tropicalis ( n=7, 1.01%). Staphylococcus aureus had a high resistance rate to penicillin G (53.57%), and Streptococcus pyogenes had a high resistance rate to tetracycline (89.47%). The resistance rate of Pseudomonas aeruginosa to furantoin was high (29.35%). The resistance rate of Klebsiella pneumoniae to ceftriaxone (38.18%) and cefzolin (36.36%) was high. The detection rate of Klebsiella pneumoniae in patients with community-acquired infection was higher than that in patients with hospital-acquired infection ( χ2=5.272, P=0.022). Conclusion:Gram-negative bacteria are the main pathogenic bacteria of skin and soft tissue infections in this area. Antibiotics should be used rationally according to the distribution of main pathogenic bacteria locally and the results of drug sensitivity.
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Objective To investigate the effect of NAIF1 in gastric cancer cell lines MKN45. Methods We constructed pLVX-Tight-Flag-NAIF1-puro plasmid with Tet-on system. DOX was added to induce NAIF1 expression in MKN45 cells. The cells were collected at 0, 6, 12 and 24 hours after DOX addition for gene expression microarray detection and biological analysis of differentially expressed genes. qRT-PCR and Western blot were used to verify the changes in mRNA and protein levels of the selected target differential genes. Results The biological analysis of gene microarray hybridization results showed that IFIT1, IFIT2 and IFIT3 expression significantly increased at 24h, qRT-PCR also showed this change, and Western blot further verified the change in protein level. However, IFIT5 showed no significant change in mRNA and gene expression. Conclusion Over-expression of NAIF1 in gastric cancer cells can promote the expression of some immune system-related IFIT family proteins.
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Objective@#To investigate the clinical value of combined detection of serum miR-378 and miR-21 in gastric cancer (GC).@*Methods@#Eighty-seven patients with GC and 78 patients with colorectal cancer(CRC) from National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences were selected, 83 individuals undergoing healthy physical examination were selected as the healthy controls. The levels of serum miR-378 and miR-21 were detected by quantitative real-time PCR (RT-qPCR) (result data were transformed as log2 for analysis).@*Results@#Relative expression levels of miR-378 in the serum were -1.24, -3.25 and -2.73 in healthy controls, GC and CRC patients, respectively. Compared with the healthy controls, the levels of serum miR-378 were significantly decreased in GC and CRC patients (both P<0.05). Relative expression levels of miR-21 in the serum were 0.11, 2.34 and 2.47 in healthy controls, GC and CRC patients, respectively. Compared with the healthy controls, the levels of serum miR-21 were significantly up-regulated in GC and CRC patients (both P<0.05). Moreover, the serum level of miR-378 in GC patients was inversely associated with tumor clinical stage (P<0.05). However, the level of miR-21 showed no significant differences among patients with different clinical and pathological characteristics (all P>0.05). The area under the receiver operating characteristic curve (AUC), sensitivity and specificity of miRNA-378 to diagnose GC was 0.770, 82.0% and 66.0%, respectively, and were 0.900, 85.0%, and 88.0% of miR-21, respectively. The AUC, sensitivity and specificity of combined detection of serum miR-378 and miR-21 to diagnose GC were 0.930, 92.0% and 87.0%, respectively, while the AUC of combined detection of serum CEA and CA-199 was 0.767, the AUC of combined all of the four factors was 0.946.@*Conclusion@#The combined detection of serum miR-378 and miR-21 have a certain effect on diagnosis of GC.